MersV1, Main, Exploration, bibRecord, 003F23

The Interwoven Architecture of the Mu Transposase Couples DNA Synapsis to Catalysis

Identifieur interne : 003F23 ( Main/Exploration ); précédent : 003F22; suivant : 003F24

The Interwoven Architecture of the Mu Transposase Couples DNA Synapsis to Catalysis

Auteurs : Hector Aldaz [États-Unis] ; Eugene Schuster [États-Unis] ; Tania A. Baker [États-Unis]

Source :

Cell [ 0092-8674 ] ; 1996.

RBID : ISTEX:365354F68B0CD54059610042AED543B886AEC623

English descriptors

Teeft :
- Acidic, Active monomer, Active protein, Active site, Active sites, Active subunit, Active subunits, Active tetramer, Active transposase, Agarose, Binding activity, Binding domain, Binding sites, Biol, Catalysis, Catalytic, Catalytic residues, Catalyze, Catalyze strand transfer, Chaconas, Chem, Cleavage, Cleavage sites, Cleavage step, Covalently, Craigie, Domain, Domain iiia, Domain iiib, Embo, Fragment, Genome, Harshey, Iiia, Inactive subunits, Inactive transposase, Integrase, Jayaram, Joinable, Joinable substrate, Mizuuchi, Monomer, Oligonucleotides, Partner substratea, Personal communication, Phage, Phage genome, Possible arrangements, Recombinase, Recombination, Recombination sites, Recombining dnas, Savilahti, Stcs, Strand, Strand transfer, Subunit, Subunit arrangement, Surette, Synapsis, Tetramer, Tetramer assembly, Tetramers, Total reaction, Total reaction mixture, Trans, Transposase, Transposase acts, Transposase subunits, Transposase tetramer, Transposition, Transposition reactions, Unjoinable, Unjoinable substrate, Unjoinable substrates, Unjoined, Unpublished data.

Abstract

Abstract: Mu transposition occurs exclusively using a pair of recombination sites found at the ends of the phage genome. To address the mechanistic basis of this specificity, we have determined both where the individual subunits of the tetrameric transposase bind on the DNA and where they catalyze DNA joining. We demonstrate that subunits do not catalyze recombination at the site adjacent to where they are bound, but rather on the opposite end of the phage genome. Furthermore, subunits bound to two different sites contribute to catalysis of one reaction step. This interwoven subunit arrangement suggests a molecular explanation for the precision with which recombination occurs using a pair of DNA signals and provides an example of the way in which the architecture of a protein–DNA complex can define the reaction products.

Url:

https://api.istex.fr/ark:/67375/6H6-04P1GBFN-C/fulltext.pdf

DOI: 10.1016/S0092-8674(00)81102-2

Affiliations:

États-Unis

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Le document en format XML

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<term>Active subunit</term>
<term>Active subunits</term>
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<term>Partner substratea</term>
<term>Personal communication</term>
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<term>Recombinase</term>
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<term>Strand transfer</term>
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<term>Synapsis</term>
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<term>Tetramer assembly</term>
<term>Tetramers</term>
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<term>Total reaction mixture</term>
<term>Trans</term>
<term>Transposase</term>
<term>Transposase acts</term>
<term>Transposase subunits</term>
<term>Transposase tetramer</term>
<term>Transposition</term>
<term>Transposition reactions</term>
<term>Unjoinable</term>
<term>Unjoinable substrate</term>
<term>Unjoinable substrates</term>
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<front><div type="abstract" xml:lang="en">Abstract: Mu transposition occurs exclusively using a pair of recombination sites found at the ends of the phage genome. To address the mechanistic basis of this specificity, we have determined both where the individual subunits of the tetrameric transposase bind on the DNA and where they catalyze DNA joining. We demonstrate that subunits do not catalyze recombination at the site adjacent to where they are bound, but rather on the opposite end of the phage genome. Furthermore, subunits bound to two different sites contribute to catalysis of one reaction step. This interwoven subunit arrangement suggests a molecular explanation for the precision with which recombination occurs using a pair of DNA signals and provides an example of the way in which the architecture of a protein–DNA complex can define the reaction products.</div>
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The Interwoven Architecture of the Mu Transposase Couples DNA Synapsis to Catalysis

The Interwoven Architecture of the Mu Transposase Couples DNA Synapsis to Catalysis

Source :

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri